5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2014.12.022

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (12): 2067-2073.doi: 10.11843/j.issn.0366-6964.2014.12.022

5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响

韩亚楠¹，杨晨玉¹，王子旭¹，陈耀星¹，孙佳毅^2,3，秦卓明⁴，曹静¹，董玉兰^1*

（1.中国农业大学动物医学院，北京 100193；2.中国农业大学生物学院，北京 100193；3.Department of Biological Sciences Purdue University，West Lafayette，IN 47907，USA；4.山东省农业科学院家禽所，济南 250100）

收稿日期:2014-04-10 出版日期:2014-12-23 发布日期:2014-12-23
通讯作者: 董玉兰， E-mail：ylbcdong@cau.edu.cn
作者简介:韩亚楠(1988-)，女，河南睢县人，硕士生，主要从事应激与肠道黏膜免疫研究，E-mail：hancau@126.com
基金资助:
863项目 (2013AA102306)；国家自然科学基金（31272483；31372332）

Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice

HAN Ya-nan¹，YANG Chen-yu¹，WANG Zi-xu¹，CHEN Yao-xing¹，SUN Jia-yi ^2,3，QIN Zhuo-ming⁴，CAO Jing¹，DONG Yu-lan ^1*

(1.College of Veterinary Medicine，China Agricultural University，Beijing 100193，China；2.College of Biology，China Agricultural University，Beijing 100193，China；3.Department of Biological Sciences Purdue University，West Lafayette,IN 47907，USA；4.Poultry institute，Shandong Academy of Agricultural Science，Jinan 250100，China)

Received:2014-04-10 Online:2014-12-23 Published:2014-12-23

摘要/Abstract

摘要：

旨在探究机体中5-HT水平与应激腹泻导致的肠道氧化应激的相关性，进一步揭示5-HT在胃肠道功能中的作用。将40只21日龄断奶仔鼠分成对照组、应激腹泻组、PCPA注射对照组（阻断5-HT合成）和PCPA注射腹泻组，利用ELISA和生物化学的方法检测5-HT和氧化产物、抗氧化酶的含量。结果表明：（1）仔鼠断奶应激腹泻导致血浆5-HT含量显著升高，比对照组升高23.42%（P=0.009）；PCPA腹腔注射对正常小鼠血浆5-HT含量影响不显著（P=0.200），但是能够有效阻断应激腹泻时5-HT的合成（P=0.006）；（2）与对照组相比，应激腹泻时不同肠段组织氧化产物（MDA）水平均显著增加，尤其十二指肠及空肠增加明显，分别升高了2.70倍（P=0.000）、3.06倍（P=0.000）；各肠段GSH-PX、CAT、SOD及T-AOC抗氧化酶水平均出现不同程度降低，十二指肠GSH-PX降低了70.59%（P=0.000），SOD活性降低了59.15%（P=0.001），T-AOC下降53.01%（P=0.000）；空肠SOD活性下降明显，降低71.05%（P=0.000）；回肠GSH-PX、CAT、SOD水平分别下降72.33%（P=0.000）、50.82%（P=0.000）、73.86%（P=0.000）；结肠CAT水平降低显著，由正常水平（0.85±0.06）U•mg^－1降低至（0.46±0.09）U•mg^－1，下降72.60%（P=0.000）；（3）5-HT合成阻断后，应激腹泻组不同肠段组织抗氧化功能均有不同程度恢复，但与正常组相比仍差异显著。结果提示应激腹泻造成肠道抗氧化功能严重损伤，而阻断5-HT合成能有效改善其抗氧化功能，从而减轻腹泻病理程度。

Abstract:

This study aimed to explore the relationship between 5-HT level and antioxidant function in the weaning stress-diarrhea intestine and further revealed the role of 5-HT in the gastrointestinal tract function.Forty 21-day-old weaned mice were divided into four treatment groups：Control，PCPA Control (block the synthesis of 5-HT)，Stress-diarrhea and PCPA+ stress-diarrhea.Plasma 5-HT was determined using ELISA kit.Intestinal oxidation products and antioxidant enzyme activity were determined by spectrophotometry.The results showed：(1) Weaning stress-diarrhea significantly increased plasma 5-HT concentration by 23.42% (P=0.009) compared with control group.Pretreated with PCPA decreased，plasma 5-HT concentration which had no significant difference in normal mice (P=0.200)，yet totally prevented the increase in 5-HT levels in weaning stress-diarrheal mice (P=0.006).(2) Oxidative product (MDA) level was significantly increased in intestine of stress-diarrhea group，and it increased by 2.70 time and 3.06 time than that of control group especially in duodenum and jejunum (P=0.000)；GSH-PX，CAT，SOD and T-AOC activities appeared decrease in a different extent in intestine of stress-diarrhea group.GSH-PX，SOD and T-AOC activities in duodenum were respectively reduced by 70.59% (P=0.000)，59.15% (P=0.001) and 53.01% (P=0.000).SOD activity in jejunum was significantly reduced by 71.05% (P=0.000) compared with that of control group.GSH-PX，CAT and SOD activities in ileum were respectively reduced by 72.33% (P=0.000)，50.82% (P=0.000)，73.86% (P=0.000).CAT activity in colon decreased significantly to 0.46±0.09 U•mg^－1 (from 0.85±0.06 U•mg^－1).(3) Pretreated with PCPA in the weaning stress-diarrheal mice could promote the intestinal antioxidant function，but this did not bring the mice to recovery.The results suggested that weaning stress-diarrhea could cause serious injury of intestinal antioxidant function，while blocking the synthesis of 5-HT could effectively improve its antioxidant function and relieve diarrhea symptom.

中图分类号:

S852.33

韩亚楠，杨晨玉，王子旭，陈耀星，孙佳毅，秦卓明，曹静，董玉兰. 5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响[J]. 畜牧兽医学报, 2014, 45(12): 2067-2073.

HAN Ya-nan，YANG Chen-yu，WANG Zi-xu，CHEN Yao-xing，SUN Jia-yi,QIN Zhuo-ming，CAO Jing，DONG Yu-lan . Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2067-2073.

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5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响

Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice

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